Coding

Part:BBa_K3839027:Design

Designed by: Georgios Psarias   Group: iGEM21_Patras   (2021-10-21)


POR-218 Transcript (CPR)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 278
    Illegal PstI site found at 475
    Illegal PstI site found at 860
    Illegal PstI site found at 1300
    Illegal PstI site found at 1360
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 278
    Illegal PstI site found at 475
    Illegal PstI site found at 860
    Illegal PstI site found at 1300
    Illegal PstI site found at 1360
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 278
    Illegal PstI site found at 475
    Illegal PstI site found at 860
    Illegal PstI site found at 1300
    Illegal PstI site found at 1360
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 278
    Illegal PstI site found at 475
    Illegal PstI site found at 860
    Illegal PstI site found at 1300
    Illegal PstI site found at 1360
    Illegal NgoMIV site found at 681
    Illegal NgoMIV site found at 800
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 115
    Illegal BsaI.rc site found at 437
    Illegal BsaI.rc site found at 1444
    Illegal SapI.rc site found at 807


Design Notes

Source

GRCh38 as a reference genome, Ensembl


References